Viperin inhibits hepatitis C virus replication by interfering with binding of NS5A to host protein hVAP-33

• DOI: 10.1099/vir.0.033860-0
• Publication Date: 2011-09-29T01:38:19Z
• Authors: Shanshan Wang; Xianfang Wu; Tingting Pan; Wuhui Song; Yaohui Wang; Fei Zhang; Zhenghong Yuan
• Keywords: Replicon; Immunoprecipitation; NS5A; Host factors; Antiviral protein

Viperin is a type-I and -II interferon-inducible intracytoplasmic protein that mediates antiviral activity against several viruses. A previous study has reported viperin could limit hepatitis C virus (HCV) replication in vitro . However, the underlying mechanism remains elusive. In present study, we found overexpression of inhibit HCV dose-dependent manner both replicon HCVcc systems. Furthermore, through co-immunoprecipitation laser confocal microscopic analysis, was to interact with host hVAP-33. Mutagenesis analysis demonstrated anti-HCV located its terminus, which required for interaction C-terminal domain Competitive showed competitively hVAP-33, therefore interfere interactions NS5A. summary, these findings suggest novel by inhibits replication, possibly binding hVAP-33 interfering