Complete molecular genome analyses of equine rotavirus A strains from different continents reveal several novel genotypes and a largely conserved genotype constellation

• DOI: 10.1099/vir.0.039255-0
• Publication Date: 2011-12-22T05:10:28Z
• Authors: Jelle Matthijnssens; Samuel Miño; Hajnalka Papp; Christiaan Potgieter; Luis Novo; Elisabeth Heylen; Mark Zeller; Lorena Garaicoechea; Alejandra Badaracco; György Lengyel; Péter Kisfali; Ann Cullinane; P. J. Collins; Max Ciarlet; Helen O’Shea; Viviana Parreño; Krisztián Bányai; María Barrandeguy; Marc Van Ranst
• Keywords: Reassortment

In this study, the complete genome sequences of seven equine group A rotavirus (RVA) strains (RVA/Horse-tc/GBR/L338/1991/G13P[18], RVA/Horse-wt/IRL/03V04954/2003/G3P[12] and RVA/Horse-wt/IRL/04V2024/2004/G14P[12] from Europe; RVA/Horse-wt/ARG/E30/1993/G3P[12], RVA/Horse-wt/ARG/E403/2006/G14P[12] RVA/Horse-wt/ARG/E4040/2008/G14P[12] Argentina; RVA/Horse-wt/ZAF/EqRV-SA1/2006/G14P[12] South Africa) were determined. Multiple novel genotypes identified genotype numbers assigned by Rotavirus Classification Working Group: R9 (VP1), C9 (VP2), N9 (NSP2), T12 (NSP3), E14 (NSP4), H7 H11 (NSP5). The constellation L338 was unique: G13-P[18]-I6-R9-C9-M6-A6-N9-T12-E14-H11. six remaining RVA showed a largely conserved constellation: G3/G14-P[12]-I2/I6-R2-C2-M3-A10-N2-T3-E2/E12-H7, which is highly divergent other known non-equine constellations. Phylogenetic analyses revealed that these are related distantly to strains, at least three lineages exist within strains. small number reassortment events observed. Interestingly, Argentina possessed E12 genotype, whereas Ireland Africa E2 genotype. unusual has until now only been described in among collected guanaco, cattle horses, suggesting geographical isolation NSP4 This genetic configuration could be useful for future vaccine development or improvement currently used vaccines.