Structure of the dengue virus glycoprotein non-structural protein 1 by electron microscopy and single-particle analysis

• DOI: 10.1099/vir.0.039321-0
• Publication Date: 2012-01-12T04:10:41Z
• Authors: David A. Muller; Michael J. Landsberg; Cheryl Bletchly; Rosalba Rothnagel; Lynne Waddington; Ben Hankamer; Paul R. Young
• Keywords: Random hexamer; Flavivirus; Structural protein; Antibody-dependent enhancement

The flavivirus non-structural protein 1 (NS1) is a glycoprotein that secreted as soluble hexameric complex during the course of natural infection. Growing evidence indicates this form NS1 (sNS1) plays significant role in immune evasion and modulation Attempts to determine crystal structure have been unsuccessful date relatively little known about macromolecular organization sNS1 hexamer. Here, we applied single-particle analysis images baculovirus-derived recombinant dengue 2 virus obtained by electron microscopy its 3D resolution 23 Å. This reveals barrel-like three dimeric units comprise hexamer provides further insights into overall oligomeric sNS1.