Specific cell tropism and neutralization of human parechovirus types 1 and 3: implications for pathogenesis and therapy development

• DOI: 10.1099/vir.0.043323-0
• Publication Date: 2012-07-27T07:43:43Z
• Authors: Brenda M. Westerhuis; Gerrit Koen; Joanne G. Wildenb...; Dasja Pajkrt; Menno D. de Jong; Kimberley S. M. B...; Katja C. Wolthers
• Keywords: Vero cell

Human parechoviruses (HPeVs) are picornaviruses frequently infecting humans. While HPeV1 is associated with mild disease, HPeV3 the cause of neonatal sepsis and meningitis. To test whether in vitro replication kinetics could be related to pathogenicity, strains isolated from patients were cultured on cell lines gastrointestinal, respiratory neural origin, measured by real-time PCR. No relationship was found between clinical symptoms strains. In contrast, showed faster cells there a higher neuropathogenicity patient. Furthermore, neutralized efficiently its specific antibody intravenous immunoglobulins (IVIG), while most not neutralized. IVIG, very low neutralizing (nAb) titres against found. Additionally, nAb observed sera two HPeV3-infected donors, high detected. Our data suggest that course infection primarily influenced strong responses, might difficult neutralize vivo therefore will mainly determined tropism.