Assembly into single-shelled virus-like particles by major capsid protein VP1 encoded by genome segment S1 of Bombyx mori cypovirus 1

• DOI: 10.1099/vir.0.19216-0
• Publication Date: 2003-08-14T23:18:49Z
• Authors: Kyoji Hagiwara; Hisashi Naitow
• Keywords: 0301 basic medicine; Microscopy, Electron; 0303 health sciences; 03 medical and health sciences; Capsid Proteins; Genome, Viral; Hydrogen-Ion Concentration; Protein Engineering; Reoviridae; Baculoviridae; Recombinant Proteins; 3. Good health

The major capsid protein VP1 encoded by genome segment S1 of Bombyx mori cypovirus 1 was expressed in a baculovirus system. In the absence of any other capsid proteins, VP1 was found to assemble into single-shelled virus-like particles. The VP1 particles were more sensitive to acidic conditions than were intact particles.