The ultrastructure of the developing replication site in foot-and-mouth disease virus-infected BHK-38 cells

• DOI: 10.1099/vir.0.19408-0
• Publication Date: 2004-03-23T19:42:56Z
• Authors: Paul Monaghan; Hannah Cook; Terry Jackson; Martin Ryan; Tom Wileman
• Keywords: Picornaviridae; Picornavirus; Aphthovirus; Echovirus

Foot-and-mouth disease virus (FMDV) is the type species of Aphthovirus genus Picornaviridae: Infection by picornaviruses results in a major rearrangement host cell membranes to create vesicular structures where genome replication takes place. In this report, using fluorescence and electron microscopy, membrane rearrangements cytoplasm FMDV-infected BHK-38 cells are documented. At 1.5-2.0 h post-infection, free ribosomes, fragmented rough endoplasmic reticulum, Golgi smooth membrane-bound vesicles accumulated on one side nucleus. Newly synthesized viral RNA was localized region cell. The changes seen distinguish from other members Picornaviridae, such as poliovirus. Firstly, collapse cellular organelles has not previously been observed for picornaviruses. Secondly, vesicles, induced FMDV, appear distinct those poliovirus echovirus 11 since they relatively few number do aggregate into densely packed clusters. Additionally, proportion with double considerably lower cells. These differences did result use study, infection these another picornavirus, bovine enterovirus (a close relative poliovirus), resulted morphological similar reported poliovirus-infected With conventional fixation, FMDV particles were seen; however, following high-pressure freezing freeze-substitution, many clusters virus-like seen.