Natural variation among human adenoviruses: genome sequence and annotation of human adenovirus serotype 1

Adenovirus genome Mastadenovirus
DOI: 10.1099/vir.0.80118-0 Publication Date: 2004-08-09T15:02:31Z
ABSTRACT
The 36,001 base pair DNA sequence of human adenovirus serotype 1 (HAdV-1) has been determined, using a 'leveraged primer sequencing strategy' to generate high quality sequences economically. This annotated genome (GenBank AF534906) confirms anticipated similarity closely related species C (formerly subgroup), adenoviruses HAdV-2 and -5, near identity with earlier reports representing parts the HAdV-1 genome. A first round data acquisition used PCR amplification primers from common genomes -5. subsequent rounds derived newly generated data. Corroborative re-sequencing selected this dataset sparsely tiled arrays ladders spanning both complementary strands These strategies allow for rapid accurate low-pass genomes. Such determinations facilitate development specific probes differentiation family, serotype, subtype strain (e.g. pathogen signatures). will be monitor epidemic outbreaks acute respiratory disease in defined test bed by Epidemic Outbreak Surveillance (EOS) project.
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