Effects of human papillomavirus type 16 oncoproteins on survivin gene expression

• DOI: 10.1099/vir.0.81067-0
• Publication Date: 2006-01-23T21:02:31Z
• Authors: Ágnes A. Borbély; Melinda Murvai; József Kónya; Zoltán Beck; Lajos Gergely; Fengzhi Li; György Veress
• Keywords: Survivin; Inhibitor of apoptosis

Survivin has recently been identified as a novel member of the inhibitor apoptosis (IAP) gene family. The product this not only suppresses but also controls cell division. is undetectable in most terminally differentiated normal tissues expressed embryonic and fetal organs present malignant tumours. Human papillomaviruses (HPV) are thought to play an important role development cervical cancer. By interfering cycle, viral oncoproteins (E6 E7) can induce immortalization host cell. transcriptional effects HPV-16 E6 E7 proteins on survivin promoter transiently transfected lines using luciferase tests were examined. E6, E7, was found significantly transactivate promoter. Experiments performed different cancer with mutants indicated that effect largely dependent p53 status. In accordance this, tumour suppressor protein downregulated expression survivin. As able interact induces its ubiquitin-dependent degradation, it appears transactivation mediated by degradation pathway. Transduction into human fibroblast cells showed HPV upregulate endogenous mRNA. Importantly, cycle synchronization experiments transcription independent cycle.