Multiple virus resistance at a high frequency using a single transgene construct

• DOI: 10.1099/vir.0.82276-0
• Publication Date: 2006-11-10T18:38:30Z
• Authors: Etienne Bucher; Dick Lohuis; Pieter M. J. A. v...; Christina Geerts-...; Rob Goldbach; Marcel Prins
• Keywords: RNA Silencing

RNA silencing is a natural antiviral defence in plants, which can be exploited transgenic plants for preprogramming virus recognition and ensuring enhanced resistance. By arranging viral transgenes as inverted repeats it thus possible to obtain strong repression of incoming viruses. Due the high sequence specificity silencing, this technology has hitherto been limited targeting single Here shown that efficient simultaneous four different tospoviruses achieved by using small transgene based on production minimal sized chimaeric cassettes. demonstrated specific siRNA accumulation, expression these cassettes rendered up 82 % transformed plant lines heritably resistant against all Thus further improved frequency multiple resistance combining fragments from series target