Soluble 3-O-sulfated heparan sulfate can trigger herpes simplex virus type 1 entry into resistant Chinese hamster ovary (CHO-K1) cells

• DOI: 10.1099/vir.0.82476-0
• Publication Date: 2007-03-20T19:23:13Z
• Authors: Vaibhav Tiwari; Christopher O'Don...; Ronald J. Copeland; Tanya Scarlett; Jian Liu; Deepak Shukla
• Keywords: 0301 basic medicine; CHO Cells; Herpesvirus 1, Human; Virus Internalization; 3. Good health; Cell Fusion; 03 medical and health sciences; Cricetulus; Cricetinae; Animals; Receptors, Virus; Heparitin Sulfate; Sulfotransferases; Glycosaminoglycans

Herpes simplex virus type 1 (HSV-1) interaction with glycoprotein D (gD) receptors facilitates entry into cells. Chinese hamster ovary (CHO-K1) cells lacking cellular allow to attach, but not enter, implying a role for during the post-attachment (entry) phase of HSV-1 infection. Here, it is shown that presence soluble heparan sulfate (HS) modified by 3-O-sulfotransferase-3 (3-OST-3), 3-OST-1, triggered resistant CHO-K1 It was further demonstrated mutant deficient in glycosaminoglycan synthesis became susceptible when spinoculated 3-OST-3-modified HS, indicating gD receptor trigger rather than cell attachment. In separate experiments, HS also fusion glycoprotein-expressing Taken together, these results show association surface-bound essential and spread.