Phosphatase PP2A and microtubule pulling forces disassemble centrosomes during mitotic exit

• DOI: 10.1101/182618
• Publication Date: 2017-08-31T05:10:23Z
• Authors: Stephen J. Enos; Martin Dressler; Beatriz Ferreira ...; Anthony A. Hyman; Jeffrey B. Woodruff
• Keywords: Centriole; Centrosome cycle; Mitotic exit

ABSTRACT Centrosomes are major microtubule-nucleating organelles that facilitate chromosome segregation and cell division in metazoans. comprise centrioles organize a micron-scale mass of protein called pericentriolar material (PCM) from which microtubules nucleate. During each cycle, PCM accumulates around through phosphorylation-mediated assembly scaffold proteins. mitotic exit, swiftly disassembles by an unknown mechanism. Here, we used Caenorhabditis elegans embryos to determine the mechanism importance disassembly dividing cells. We found phosphatase PP2A its regulatory subunit SUR-6 (PP2A ), together with cortically directed microtubule pulling forces, actively disassemble PCM. In depleted these activities, ~25% persisted one cycle into next, resulting cytokinetic furrow ingression errors, excessive centrosome accumulation, embryonic death. Purified pp2A could dephosphorylate SPD-5 vitro . Our data suggest occurs combination dephosphorylation components catastrophic rupture scaffold.