Toward an integrated classification of neuronal cell types: morphoelectric and transcriptomic characterization of individual GABAergic cortical neurons

• DOI: 10.1101/2020.02.03.932244
• Publication Date: 2020-02-04T19:49:25Z
• Authors: Nathan W. Gouwens; Staci A. Sorensen; Fahimeh Baftizadeh; Agata Budzillo; Brian R. Lee; Tim Jarsky; Lauren Alfiler; Anton Arkhipov; Katherine Baker; Eliza Barkan; Kyla Berry; Darren Bertagnolli; Kris Bickley; Jasmine Bomben; Thomas Braun; Krissy Brouner; Tamara Casper; Kirsten Crichton; Tanya L. Daigle; Rachel Dalley; Rebecca de Frates; Nick Dee; Tsega Desta; Samuel Dingman Lee; Nadezhda Dotson; Tom Egdorf; Lauren Ellingwood; Rachel Enstrom; Luke Esposito; Colin Farrell; David Feng; Olivia Fong; Rohan Gala; Clare Gamlin; Amanda Gary; Alexandra Glandon; Jeff Goldy; Melissa Gorham; Lucas Graybuck; Hong Gu; Kristen Hadley; Michael J. Hawrylycz; Alex M. Henry; DiJon Hill; Madie Hupp; Sara Kebede; Tae Kyung Kim; Lisa Kim; Matthew Kroll; Changkyu Lee; Katherine E. Link; Matthew Mallory; Rusty Mann; Michelle Maxwell; Medea McGraw; Delissa McMillen; Alice Mukora; Lindsay Ng; Lydia Ng; Kiet Ngo; Philip R. Nicovich; Aaron Oldre; Daniel Park; Hanchuan Peng; Osnat Penn; Thanh Pham; Alice Pom; Lydia Potekhina; Ramkumar Rajanbabu; Shea Ransford; David Reid; Christine Rimorin; Miranda Robertson; Kara Ronellenfitch; Augustin Ruiz; David Sandman; Kimberly Smith; Josef Sulc; Susan M. Sunkin; Aaron Szafer; Michael Tieu; Amy Torkelson; Jessica Trinh; Herman Tung; Wayne Wakeman; Katelyn Ward; Grace Williams; Zhi Zhou; Jonathan Ting; Uygar Sumbul; Ed Lein; Christof Koch; Zizhen Yao; Bosiljka Tasic; Jim Berg; Gabe J. Murphy; Hongkui Zeng
• Keywords: 0301 basic medicine; 03 medical and health sciences

AbstractNeurons are frequently classified into distinct groups or cell types on the basis of structural, physiological, or genetic attributes. To better constrain the definition of neuronal cell types, we characterized the transcriptomes and intrinsic physiological properties of over 3,700 GABAergic mouse visual cortical neurons and reconstructed the local morphologies of 350 of those neurons. We found that most transcriptomic types (t-types) occupy specific laminar positions within mouse visual cortex, and many of those t-types exhibit consistent electrophysiological and morphological features. We observed that these properties could vary continuously between t-types, which limited the ability to predict specific t-types from other data modalities. Despite that, the data support the presence of at least 20 interneuron met-types that have congruent morphological, electrophysiological, and transcriptomic properties.HighlightsPatch-seq data obtained from>3,700 GABAergic cortical interneuronsComprehensive characterization of morpho-electric features of transcriptomic types20 interneuron met-types that have congruent properties across data modalitiesDifferent Sst met-types preferentially innervate different cortical layers