Enhancing Colorimetric LAMP Amplification Speed and Sensitivity with Guanidine Chloride

• DOI: 10.1101/2020.06.03.132894
• Publication Date: 2020-06-05T10:55:16Z
• Authors: Yinhua Zhang; Guoping Ren; Jackson Buss; Andrew J. Barry; Gregory C. Patton; Nathan A. Tanner
• Keywords: 0303 health sciences; 03 medical and health sciences; 3. Good health

AbstractLoop-mediated isothermal amplification (LAMP) is a versatile technique for detection of target DNA and RNA, enabling rapid molecular diagnostic assays with minimal equipment. The global SARS-CoV-2 pandemic has presented an urgent need for new and better diagnostic methods, with colorimetric LAMP utilized in numerous studies for SARS-CoV-2 detection. However, the sensitivity of colorimetric LAMP in early reports has been below that of the standard RT-qPCR tests, and we sought to improve performance. Here we report the use of guanidine hydrochloride and combined primer sets to increase speed and sensitivity in colorimetric LAMP, bringing this simple method up to the standards of sophisticated technique and enabling accurate and high-throughput diagnostics.