Evaluation of the immunogenicity of prime-boost vaccination with the replication-deficient viral vectored COVID-19 vaccine candidate ChAdOx1 nCoV-19

• DOI: 10.1101/2020.06.20.159715
• Publication Date: 2020-06-21T04:27:10Z
• Authors: Simon P. Graham; Rebecca K. McLean; Alexandra J. Spencer; Sandra Belij-Ramm...; Daniel Wright; Marta Ulaszewska; Jane C. Edwards; Jack W. P. Hayes; Veronica Martini; Nazia Thakur; Carina Conceicao; Isabelle Dietrich; Holly Shelton; Ryan Waters; Anna Ludi; Ginette Wilsden; Clare Browning; Dagmara Bialy; Sushant Bhat; Phoebe Stevenson-...; Philippa Hollingh...; Ciaran Gilbride; David Pulido; Katy Moffat; Hannah Sharpe; Elizabeth Allen; Valerie Mioulet; Chris Chiu; Joseph Newman; Amin S. Asfor; Alison Burman; Sylvia Crossley; Jiandong Huo; Raymond J. Owens; Miles Carroll; John A. Hammond; Elma Tchilian; Dalan Bailey; Bryan Charleston; Sarah C. Gilbert; Tobias J. Tuthill; Teresa Lambe
• Keywords: 2019-20 coronavirus outbreak

Abstract Clinical development of the COVID-19 vaccine candidate ChAdOx1 nCoV-19, a replication-deficient simian adenoviral vector expressing full-length SARS-CoV-2 spike (S) protein was initiated in April 2020 following non-human primate studies using single immunisation. Here, we compared immunogenicity one or two doses nCoV-19 both mice and pigs. Whilst dose induced antigen-specific antibody T cells responses, booster immunisation enhanced particularly pigs, with significant increase neutralising titres.