Real-Time Multi-Angle Projection Imaging of Biological Dynamics
Male
Neurons
0301 basic medicine
0303 health sciences
Embryo, Nonmammalian
Microscopy, Confocal
Colon
Heart
Mice, Transgenic
Rats
Rats, Sprague-Dawley
Mice
03 medical and health sciences
Imaging, Three-Dimensional
Spheroids, Cellular
Image Processing, Computer-Assisted
Animals
Humans
Female
Zebrafish
DOI:
10.1101/2020.10.29.355917
Publication Date:
2020-10-30T08:15:13Z
AUTHORS (12)
ABSTRACT
AbstractWe introduce a cost-effective and easily implemented scan unit which enables any camera-based microscope to perform projection imaging from diverse viewing angles. We demonstrate this capability on Lattice Light-Sheet and Oblique Plane Microscopy by rapidly delivering projection images with an uncompromised lateral resolution and high optical contrast. By imaging the sample from one or multiple perspectives, our method enables visualization of rapid biological processes, real time stereoscopic imaging as well as three-dimensional particle localization throughout a cellular volume from just two images. Furthermore, because our projection imaging technique provides intuitive three-dimensional renderings in real-time, it improves microscope usability, allows users to more-readily optimize instrument performance and identify biological phenomena of interest on-the-fly, while also reducing data overhead by a factor of >100. We leverage our rapid projection method to image cancer cell morpho-dynamics and calcium signaling in cultured neurons, to perform three-dimensional localization of genetically encoded nanoparticles, as well as to image orthogonal views of an embryonic Zebrafish heart simultaneously.
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CITATIONS (1)
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