Real-Time Multi-Angle Projection Imaging of Biological Dynamics

Male Neurons 0301 basic medicine 0303 health sciences Embryo, Nonmammalian Microscopy, Confocal Colon Heart Mice, Transgenic Rats Rats, Sprague-Dawley Mice 03 medical and health sciences Imaging, Three-Dimensional Spheroids, Cellular Image Processing, Computer-Assisted Animals Humans Female Zebrafish
DOI: 10.1101/2020.10.29.355917 Publication Date: 2020-10-30T08:15:13Z
ABSTRACT
AbstractWe introduce a cost-effective and easily implemented scan unit which enables any camera-based microscope to perform projection imaging from diverse viewing angles. We demonstrate this capability on Lattice Light-Sheet and Oblique Plane Microscopy by rapidly delivering projection images with an uncompromised lateral resolution and high optical contrast. By imaging the sample from one or multiple perspectives, our method enables visualization of rapid biological processes, real time stereoscopic imaging as well as three-dimensional particle localization throughout a cellular volume from just two images. Furthermore, because our projection imaging technique provides intuitive three-dimensional renderings in real-time, it improves microscope usability, allows users to more-readily optimize instrument performance and identify biological phenomena of interest on-the-fly, while also reducing data overhead by a factor of >100. We leverage our rapid projection method to image cancer cell morpho-dynamics and calcium signaling in cultured neurons, to perform three-dimensional localization of genetically encoded nanoparticles, as well as to image orthogonal views of an embryonic Zebrafish heart simultaneously.
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