Multidimensional Single-Nuclei RNA-Seq Reconstruction of Adipose Tissue Reveals Adipocyte Plasticity Underlying Thermogenic Response

• DOI: 10.1101/2021.02.16.431320
• Publication Date: 2021-02-18T02:21:38Z
• Authors: Carlos Alberto Ol...; Sarah Santiloni Cury; Cleidson de Pádua...; Nabil Rabhi; Wilson Araujo Silva; Stephen R. Farmer; Robson Francisco ...; Miguel Luiz Batista
• Keywords: Cell Nucleus; 0301 basic medicine; Mature adipocyte plasticity; QH573-671; mature adipocyte plasticity; cellular compartment prediction; Adipose Tissue, White; Adipocytes, White; Cell Plasticity; Adipocyte subpopulations; Temperature; Thermogenesis; Article; Mice; 03 medical and health sciences; Thermogenic treatment; adipocyte subpopulations; thermogenic treatment; Transcriptional factors; Animals; transcriptional factors; RNA-Seq; Cytology; Uncoupling Protein 1; Cellular compartment prediction

Abstract Adipose tissue has been classified based on its morphology and function as white, brown, or beige / brite. It plays an essential role a regulator of systemic metabolism through paracrine endocrine signals. Recently, multiple adipocyte subtypes have revealed using RNA sequencing technology, going beyond simply defined but by their cellular origin, adaptation to metabolic stress, plasticity. Here, we performed in-depth analysis publicly available single-nuclei RNAseq from adipose utilized workflow template characterize plasticity, heterogeneity, secretome profiles. The reanalyzed dataset led the identification different adipocytes including three subpopulations thermogenic provided characterization distinct transcriptional profiles along trajectory under challenges. This study provides useful resource for further investigations regarding mechanisms related plasticity trans-differentiation. Highlights Multidimensional transcriptome at single-nucleus resolution recovers nuclei cell types in Adaptative response results 3 mature Single-nuclei transcriptomic-based reveals cell-type-specific genes vivo sets trans-differentiation Graphic