Multidimensional Single-Nuclei RNA-Seq Reconstruction of Adipose Tissue Reveals Adipocyte Plasticity Underlying Thermogenic Response
Cell Nucleus
0301 basic medicine
Mature adipocyte plasticity
QH573-671
mature adipocyte plasticity
cellular compartment prediction
Adipose Tissue, White
Adipocytes, White
Cell Plasticity
Adipocyte subpopulations
Temperature
Thermogenesis
Article
Mice
03 medical and health sciences
Thermogenic treatment
adipocyte subpopulations
thermogenic treatment
Transcriptional factors
Animals
transcriptional factors
RNA-Seq
Cytology
Uncoupling Protein 1
Cellular compartment prediction
DOI:
10.1101/2021.02.16.431320
Publication Date:
2021-02-18T02:21:38Z
AUTHORS (8)
ABSTRACT
AbstractAdipose tissue has been classified based on its morphology and function as white, brown, or beige / brite. It plays an essential role as a regulator of systemic metabolism through paracrine and endocrine signals. Recently, multiple adipocyte subtypes have been revealed using RNA sequencing technology, going beyond simply defined morphology but by their cellular origin, adaptation to metabolic stress, and plasticity. Here, we performed an in-depth analysis of publicly available single-nuclei RNAseq from adipose tissue and utilized a workflow template to characterize adipocyte plasticity, heterogeneity, and secretome profiles. The reanalyzed dataset led to the identification of different subtypes of adipocytes including three subpopulations of thermogenic adipocytes and provided a characterization of distinct transcriptional profiles along the adipocyte trajectory under thermogenic challenges. This study provides a useful resource for further investigations regarding mechanisms related to adipocyte plasticity and trans-differentiation.HighlightsMultidimensional transcriptome analysis at single-nucleus resolution recovers nuclei of cell types in adipose tissueAdaptative thermogenic response results in 3 distinct mature adipose cell typesSingle-nuclei transcriptomic-based secretome analysis reveals adipose cell-type-specific genesThein vivotrajectory of adipocyte plasticity for thermogenic response reveals sets of trans-differentiation genesGraphic Abstract
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CITATIONS (1)
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