Multidimensional Single-Nuclei RNA-Seq Reconstruction of Adipose Tissue Reveals Adipocyte Plasticity Underlying Thermogenic Response

Cell Nucleus 0301 basic medicine Mature adipocyte plasticity QH573-671 mature adipocyte plasticity cellular compartment prediction Adipose Tissue, White Adipocytes, White Cell Plasticity Adipocyte subpopulations Temperature Thermogenesis Article Mice 03 medical and health sciences Thermogenic treatment adipocyte subpopulations thermogenic treatment Transcriptional factors Animals transcriptional factors RNA-Seq Cytology Uncoupling Protein 1 Cellular compartment prediction
DOI: 10.1101/2021.02.16.431320 Publication Date: 2021-02-18T02:21:38Z
ABSTRACT
AbstractAdipose tissue has been classified based on its morphology and function as white, brown, or beige / brite. It plays an essential role as a regulator of systemic metabolism through paracrine and endocrine signals. Recently, multiple adipocyte subtypes have been revealed using RNA sequencing technology, going beyond simply defined morphology but by their cellular origin, adaptation to metabolic stress, and plasticity. Here, we performed an in-depth analysis of publicly available single-nuclei RNAseq from adipose tissue and utilized a workflow template to characterize adipocyte plasticity, heterogeneity, and secretome profiles. The reanalyzed dataset led to the identification of different subtypes of adipocytes including three subpopulations of thermogenic adipocytes and provided a characterization of distinct transcriptional profiles along the adipocyte trajectory under thermogenic challenges. This study provides a useful resource for further investigations regarding mechanisms related to adipocyte plasticity and trans-differentiation.HighlightsMultidimensional transcriptome analysis at single-nucleus resolution recovers nuclei of cell types in adipose tissueAdaptative thermogenic response results in 3 distinct mature adipose cell typesSingle-nuclei transcriptomic-based secretome analysis reveals adipose cell-type-specific genesThein vivotrajectory of adipocyte plasticity for thermogenic response reveals sets of trans-differentiation genesGraphic Abstract
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