Class 1 integrons in clinical and swine industry isolates of Salmonella Typhimurium from Colombia, dating 1997 to 2017

• DOI: 10.1101/2022.02.14.22270819
• Publication Date: 2022-02-16T07:30:20Z
• Authors: Nancy Yaneth Flór...; Elizabeth Noelia ...; Blanca Perez-Sepu...; Eyda Lizeth Ospin...; Ana Karina Carras...; Iliana C Chamorro...; Lucy Angeline Mon...; Yan Li; Silvana Zapata-Be...; Jay C.D. Hinton; José Miguel Villa...; Magdalena Wiesner
• Keywords: Salmonella enterica; Gene cassette; Multilocus sequence typing

Abstract Background Salmonella enterica subsp. serovar Typhimurium ( S. Typhimurium) has been linked to outbreaks of foodborne gastroenteritis disease, and the emergence antimicrobial-resistant clones. In Colombia, laboratory surveillance spp. between 1997-2018 revealed that S . was most ubiquitous (27.57% all isolates), with increasing levels antimicrobial resistance several families antibiotics. Hypothesis Resistant isolates recovered from clinical swine samples carry class 1 integrons are genes. Aim Identify integrons, investigate their association other mobile genetic elements, relationship Colombian isolates. Methods. this study, 392 were analyzed, which 237 obtained blood culture, 155 non-invasive sources 50 32 a slaughterhouse, 10 point sale 8 cutting plant. Class plasmid incompatibility groups analyzed by PCR Whole Genome Sequencing (WGS), region surrounding identified WGS. The phylogenetic established MLST SNP analysis. Results. Overall, 39.03% (153/392) 22% (11/50) carried complete integrons. Twelve types gene cassette arrays identified, including dfr7 - aac bla OXA-2 (Int1-Col1) as frequent in (75.2% = 115/153). Clinical resistant up 5 3 families, respectively. Int1-Col1 integron prevalent stool associated Tn21. common group IncA/C. Conclusions. widespread presence (IntI1-Col1) Colombia since 1997 striking. We speculate source elements favors dispersion antibiotic determinants