The Serological Sciences Network (SeroNet) for COVID-19: Depth and Breadth of Serology Assays and Plans for Assay Harmonization

• DOI: 10.1101/2022.02.27.22271399
• Publication Date: 2022-03-01T16:44:06Z
• Authors: Amy B. Karger; James D. Brien; Jayne M. Christen; Santosh Dhakal; Troy J. Kemp; Sabra L. Klein; Ligia A. Pinto; Lakshmanane Premk...; John D. Roback; Raquel A. Binder; Karl W. Boehme; Suresh Boppana; Carlos Cordon-Cardo; James M. Crawford; John L. Daiss; Alan P. Dupuis; Ana M. Espino; Adolfo Firpo-Beta...; Catherine Forconi; J. Craig Forrest; Roxie C. Girardin; Douglas A. Granger; Steve W. Granger; Natalie S. Haddad; Christopher D. He...; Danielle T. Hunt; Joshua L. Kennedy; Christopher L. King; Florian Krammer; Kate Kruczynski; Joshua LaBaer; F. Eun-Hyung Lee; William T. Lee; Shan-Lu Liu; Gerard Lozanski; Todd Lucas; Damodara Rao Mendu; Ann M. Moormann; Vel Murugan; Nkemakonam C. Okoye; Petraleigh Pantoja; Anne F. Payne; Jin Park; Swetha Pinninti; Amelia K. Pinto; Nora Pisanic; Ji Qiu; Carlos A. Sariol; Viviana Simon; Lusheng Song; Tara L. Steffen; E. Taylor Stone; Linda M. Styer; Mehul S. Suthar; Stefani N. Thomas; Bharat Thyagarajan; Ania Wajnberg; Jennifer L. Yates; Kimia Sobhani
• Keywords: Harmonization; 2019-20 coronavirus outbreak

In October 2020, the National Cancer Institute (NCI) Serological Sciences Network (SeroNet) was established to study immune response COVID-19, and "to develop, validate, improve, implement serological testing associated technologies." SeroNet is comprised of 25 participating research institutions partnering with Frederick Laboratory for Research (FNLCR) Coordinating Center. Since its inception, has supported collaborative development sharing COVID-19 assay procedures set forth plans harmonization.To facilitate collaboration procedure sharing, a detailed survey sent collate comprehensive details performance metrics on assays within SeroNet. addition, FNLCR protocol calibrate reference standards, such as U.S. SARS-CoV-2 serology standard material First WHO International Standard (IS) anti-SARS-CoV-2 immunoglobulin (20/136), harmonization reporting units cross-comparison data.SeroNet reported total 27 ELISA methods, 13 multiplex assays, 9 neutralization use 12 different commercial methods. developed standardized these diverse standards.SeroNet have array virus vaccines. Calibration harmonize results will future pooled data analyses cross-comparisons.