Homophilic wiring principles underpin neuronal network topologyin vitro

• DOI: 10.1101/2022.03.09.483605
• Publication Date: 2022-03-11T00:15:15Z
• Authors: Danyal Akarca; Alexander W. E. Dunn; Philipp J. Hornauer; Silvia Ronchi; Michele Fiscella; Congwei Wang; Marco Terrigno; Ravi Jagasia; Petra E. Vértes; Susanna B. Mierau; Ole Paulsen; Stephen J. Eglen; Andreas Hierlemann; Duncan E. Astle; Manuel Schröter
• Keywords: Biological neural network; Generative model

ABSTRACT Economic efficiency has been a popular explanation for how networks self-organize within the developing nervous system. However, precise nature of economic negotiations governing this putative organizational principle remains unclear. Here, we address question further by combining large-scale electrophysiological recordings, to characterize functional connectivity neuronal in vitro , with generative modeling approach capable simulating network formation. We find that best fitting model uses homophilic wiring which neurons form connections other are spatially proximal and have similar patterns themselves. Homophilic models outperform more canonical wire depending upon their spatial proximity either alone or combination extent local connectivity. This homophily-based mechanism emergence accounts wide range observations described, but not sufficiently explained, traditional analyses topology. Using rodent human monolayer organoid cultures, show mechanisms can accurately recapitulate topology emerging cellular connectivity, representing an important determining factor formation .