Sonic Hedgehog Activates Prostaglandin Signaling to Stabilize Primary Cilium Length

• DOI: 10.1101/2022.05.06.490951
• Publication Date: 2022-05-09T15:50:22Z
• Authors: Shariq S. Ansari; Miriam E. Dillard; Yan Zhang; Mary Ashley Austria; Naoko Boatwright; Elaine L. Shelton; Amanda Johnson; Brandon M. Young; Zoran Rankovic; John D. Schuetz; Camenzind G. Robi...; Stacey K. Ogden
• Keywords: Smoothened; Primary (astronomy)

ABSTRACT Sonic Hedgehog (SHH) signaling is an essential driver of embryonic patterning that, when corrupted, leads to developmental disorders and cancers. SHH effector responses are organized through nonmotile primary cilia that grow retract with the cell cycle in response distinct extracellular cues. Destabilization cilium length corrupts pathway regulation, which places significant pressure on maintain ciliary architecture. Herein, we investigate whether promotes control. We reveal a signal crosstalk circuit induced by activation Phospholipase A2 (cPLA 2 ) drives EP 4 stabilize length. demonstrate chemical or genetic blockade SHH-EP destabilized cyclic AMP (cAMP) control, reduced length, attenuated induction. Accordingly, find Ep -/- mice display shortened neuroepithelial altered SHH-dependent neuronal fate specification. Thus, initiates maintains for robust downstream response.