Transretinal migration of astrocytes and brain/spinal cord-like cells arising from transplanted human retinal organoids

• DOI: 10.1101/2022.05.12.491655
• Publication Date: 2022-05-20T08:38:47Z
• Authors: Ying V. Liu; Clayton P. Santiago; Akin Sogunro; Gregory J. Konar; Ming-wen Hu; Minda M. McNally; Yu-chen Lu; Zhuo-lin Li; Dzhalal Agakishiev; Sarah E. Hadyniak; Katarzyna A. Hussey; Tyler J. Creamer; Linda D. Orzolek; Derek Teng; Jiang Qian; Zheng Jiang; Robert J. Johnston; Seth Blackshaw; Mandeep S. Singh
• Keywords: Organoid

Abstract Human retinal organoid transplantation can potentially restore vision in patients with degenerative diseases. How the recipient retina regulates maturation, fate specification, and migration of transplanted cells is unknown. We human organoid-derived into photoreceptor-deficient mice, conducted histology single-cell RNA sequencing analyses, observed two main classes graft-derived cells. The first class consisted astrocytes brain/spinal cord-like neural precursors, absent or rare cultured organoids, that migrated all layers traveled long distances. second progenitor-derived cells, including rods cones, remained subretinal space matured more rapidly than photoreceptors culture. These data suggest promotes maturation while inducing expanding migratory cell populations are not normally derived from progenitors. findings have important implications for cell-based treatment