The TRIPLE PHD FINGERS proteins are required for SWI/SNF complex-mediated +1 nucleosome positioning and 5’ transcript length determination in Arabidopsis

• DOI: 10.1101/2022.06.12.495191
• Publication Date: 2022-06-13T03:20:10Z
• Authors: Borja Diego-Martin; Jaime Pérez-Alemany; Joan Candela-Ferre; Antonio Corbalán-...; Juan Pereyra; David Alabadí; Yasaman Jami-Alah...; James Wohlschlegel; Javier Gallego-Ba...
• Keywords: SWI/SNF

ABSTRACT Eukaryotes have evolved multiple ATP-dependent chromatin remodelers to shape the nucleosome landscape. We recently uncovered an evolutionarily conserved SWItch/Sucrose Non-Fermentable (SWI/SNF) remodeler complex in plants reminiscent of mammalian BAF subclass, which specifically incorporates MINUSCULE (MINU) catalytic subunits and TRIPLE PHD FINGERS (TPF) signature subunits. Here we report experimental evidence that establishes functional relevance TPF proteins for activity. Our results show depletion triggers similar pleiotropic phenotypes molecular defects those found minu mutants. Moreover, genomic location MINU2 as representative members plant BAF-like their impact on positioning transcription. These analyses unravel binding thousands genes where it modulates position +1 nucleosome. targets tend produce 5’-shifted transcripts tpf mutants pointing participation alternative transcriptional start site usage. Interestingly, there is a remarkable correlation between shift 5’ transcript length change suggesting connection. In summary, this study unravels function SWI/SNF involved determination.