tRNA Analysis of eXpression (tRAX): A tool for integrating analysis of tRNAs, tRNA-derived small RNAs, and tRNA modifications

• DOI: 10.1101/2022.07.02.498565
• Publication Date: 2022-07-04T06:25:11Z
• Authors: Andrew D. Holmes; Jonathan M. Howard; Patricia P. Chan; Todd M. Lowe
• Keywords: 0301 basic medicine; 03 medical and health sciences

Abstract Recent high-throughput sequencing protocols have facilitated increased accuracy in measurements of transfer tRNAs (tRNAs) and tRNA-derived small RNAs (tDRs) from biological samples. However, commonly used RNA-seq analysis pipelines overlook special considerations given the unique features tRNA metabolism. We present tRAX (tRNA Analysis eXpression), a user-friendly analytic package for streamlined processing graphic presentation small-RNA data. Here, we apply it to both tDRs mouse tissues illustrate extensive visualization features. Biologically compelling results demonstrate as an effective accessible tool in-depth characterization tDR transcriptomes.