A genome-scale atlas reveals complex interplay of transcription and translation in an archaeon

• DOI: 10.1101/2022.08.31.505529
• Publication Date: 2022-09-03T18:05:25Z
• Authors: Alan P. R. Lorenz...; Ulrike Kusebauch; Lívia S. Zaramela; Wei-Ju Wu; João P. P. de Alm...; Serdar Turkarslan; Adrián L. G. de L...; José V. Gomes-Filho; Ricardo Z. N. Vêncio; Robert L. Moritz; Tie Koide; Nitin S. Baliga
• Keywords: Post-transcriptional regulation

ABSTRACT The scale of post-transcriptional regulation and the implications its interplay with other forms on environmental acclimation is underexplored for organisms domain Archaea. Here, we have investigated in extremely halophilic archaeon Halobacterium salinarum NRC-1 by integrating transcriptome-wide locations transcript processing sites (TPS) SmAP1 binding, genome-wide antisense RNAs (asRNAs), consequences RNase_2099C knockout differential expression all genes. This integrated analysis has discovered that 54% protein-coding genes genome this haloarchaeon are likely targeted multiple mechanisms putative regulation, about 20% regulated combinatorial schemes involving SmAP1, asRNAs, RNase_2099C. Comparative mRNA levels (RNA-Seq) protein (SWATH-MS) 2,579 over four phases batch culture growth complex medium generated additional evidence conditional 7% We demonstrate may act to fine-tune specialized rapid stressful environments, e.g., as a switch turn gas vesicle biogenesis promote vertical relocation anoxic conditions modulate frequency transposition IS elements 200 /IS 605 , 4 H3 families. Findings from study provided an atlas public web resource ( https://halodata.systemsbiology.net ). IMPORTANCE While transcriptional landscape archaea been extensively investigated, currently limited knowledge driving life. In study, collected omics data sources technologies infer post-transcriptionally modulating their at level NRC-1. results suggest drive regulating hallmark biological processes. To foster discoveries research groups interested topic, extended our form interactive