Ancestral SARS-CoV-2 driven antibody repertoire diversity in an unvaccinated individual correlates with expanded neutralization breadth

• DOI: 10.1101/2022.10.19.512979
• Publication Date: 2022-10-21T05:06:20Z
• Authors: Suprit Deshpande; Mohammed Yousuf A...; Jyoti Sutar; Payel Das; Nitin Hingankar; Sohini Mukherjee; Priyanka Jayal; Savita Singh; Anbalagan Anantharaj; Janmejay Singh; Souvick Chattopad...; Sreevatsan Raghavan; Mudita Gosain; Supriya Chauhan; Shweta Shrivas; Chaman Prasad; Sangeeta Chauhan; Neha Sharma; Pradipta Jana; Ramachandran Thir...; Pallavi Kshetrapal; Nitya Wadhwa; Bhabatosh Das; Gaurav Batra; Guruprasad Medigeshi; Devin Sok; Shinjini Bhatnagar; Pramod Kumar Garg; Jayanta Bhattacharya
• Keywords: Antibody Repertoire

Abstract Understanding the quality of immune repertoire triggered during natural infection can provide vital clues that form basis for development humoral response in some individuals capable broadly neutralizing pan SARS-CoV-2 variants. We assessed diversity antibody responses developed an unvaccinated individual infected with ancestral by examining ability distinct B cell germline-derived monoclonal antibodies (mAbs) known and currently circulating Omicron variants pseudovirus authentic virus neutralization assays. The post vaccination was compared to obtained at baseline same those from breakthrough assay. Broadly mAbs representing unique lineages non-overlapping epitope specificities isolated a single donor varied their neutralize Plasma this demonstrated BA.1, BA.2 BA.4 increased magnitude found be comparable other vaccinated who were SARS-CoV-2. Development producing affinity antigen immediately after eliciting offers highest probability protecting against evolving Importance robust convalescent is known, however varies population level. early 2020 not only lineage origin but also capability potency all VOC This indicated establishment contributed forming particular following giving rise diverse could compensate each providing polyclonal response. Individuals able produce such potent have higher chance being protected