Bursting Translation on Single mRNAs in Live Cells

• DOI: 10.1101/2022.11.07.515520
• Publication Date: 2022-11-08T23:10:16Z
• Authors: Nathan M. Livingston; Jiwoong Kwon; Oliver Valera; James A. Saba; Niladri K. Sinha; Pranav Reddy; Blake Nelson; Clara Wolfe; Taekjip Ha; Rachel Green; Jian Liu; Bin Wu
• Keywords: Bursting; Translational regulation; Transcription; Live cell imaging

Summary Stochasticity has emerged as a mechanism to control gene expression. Much of this so-called “noise” been attributed bursting transcription. However, the stochasticity translation not similarly investigated due lack enabling imaging technologies. We developed techniques track single mRNAs and their in live cells for hours, allowing measurement previously uncharacterized dynamics. applied genetic pharmacological perturbations kinetics. Like transcription, is constitutive process but instead cycles between inactive active states or “bursts”. But unlike which largely frequency modulated, complex structure 5’-untranslated region alters burst amplitude. Bursting can be controlled through cap-proximal sequences trans -acting factors such eIF4F. coupled molecule with stochastic modeling deduce fundamental kinetic parameters translational bursting, new dimension control. Highlights Long-term tracking reveals multi-state, Structure modulates amplitude 5’-cap proximal modulate mTOR signaling adjusts respond environmental cues