miR-210 expression is strongly hypoxia-induced in anaplastic thyroid cancer cell lines and is associated with extracellular vesicles & Argonaute-2

• DOI: 10.1101/2022.11.23.515840
• Publication Date: 2022-11-25T05:35:11Z
• Authors: Bonita H. Powell; Andrey Turchinovich; Yongchun Wang; Olesia Gololobova; Dominik Buschmann; Martha A. Zeiger; Christopher B. Um...; Kenneth W. Witwer
• Keywords: Hypoxia; Anaplastic thyroid cancer; Extracellular vesicles; Tumor Hypoxia; Oxygen tension

ABSTRACT Hypoxia, or low oxygen tension, is frequently found in highly proliferative solid tumors such as anaplastic thyroid carcinoma (ATC) and believed to promote resistance chemotherapy radiation. Identifying hypoxic cells for targeted therapy may thus be an effective approach treating aggressive cancers. Here, we explore the potential of well-known hypoxia-responsive microRNA (miRNA) miR-210-3p a cellular extracellular biological marker hypoxia. We compare miRNA expression across several ATC papillary cancer (PTC) cell lines. In line SW1736, levels indicate hypoxia during exposure conditions (2% O 2 ). Furthermore, when released by SW1736 into space, associated with RNA carriers vesicles (EVs) Argonaute-2 (AGO2), making it