Spatial transcriptome profiling ofin vitro3D tumouroids to study tumour-stroma interactions

• DOI: 10.1101/2022.12.13.520130
• Publication Date: 2022-12-15T20:15:27Z
• Authors: Deniz Bakkalci; Georgina Al-Badri; Wei Yang; Andy Nam; Yan Liang; Syed Ali Khurram; Susan Heavey; Stefano Fedele; Umber Cheema
• Keywords: 0301 basic medicine; 03 medical and health sciences

Abstract Bioengineering facets of the tumour microenvironment (TME) are essential in 3D tissue models to accurately recapitulate progression. Stromal cells key components TME and their incorporation into biomimetic bioengineered tumour-stroma is be able mimic TME. By engineering tumouroids with distinct stromal compartments, it has been possible identify how gene expression altered by presence different using spatial transcriptomics. Ameloblastoma a benign epithelial jawbone engineered multi-compartment increased oncogenes was found where osteoblasts (bone stroma) were present. Engineering gingival fibroblast stroma resulted matrix remodelling genes ameloblastoma tumour. This study provides evidence show specific effect on behaviour illustrates importance biologically relevant for models. Our novel results that an causes upregulation which directly correlates measured invasion model. In contrast osteoblast/bone increases cells.