Sexual dimorphism and the multi-omic response to exercise training in rat subcutaneous white adipose tissue

• DOI: 10.1101/2023.02.03.527012
• Publication Date: 2023-02-05T05:30:10Z
• Authors: Gina M. Many; James A. Sanford; Tyler J. Sagendorf; Zhenxin Hou; Pasquale Nigro; Katie Whytock; David Amar; Tiziana Caputo; Nicole R. Gay; David A. Gaul; Michael Hirshman; David Jimenez-Mor...; Malene E. Lindholm; Michael J. Muehlb...; Maria Vamvini; Bryan Bergman; Facundo M. Fernández; Laurie J. Goodyear; Eric A. Ortlund; Lauren M. Sparks; Ashley Xia; Joshua N. Adkins; Sue C. Bodine; Christopher B. Ne...; Simon Schenk
• Keywords: Sexual dimorphism; Endurance Training; Omics

Abstract Subcutaneous white adipose tissue (scWAT) is a dynamic storage and secretory organ that regulates systemic homeostasis, yet the impact of endurance exercise training sex on its molecular landscape has not been fully established. Utilizing an integrative multi-omics approach with data generated by Molecular Transducers Physical Activity Consortium (MoTrPAC), we identified profound sexual dimorphism in response rat scWAT to training. Despite similar cardiorespiratory improvements, only male rats reduced whole-body adiposity, adipocyte size, total triglyceride abundance Multi-omic analyses integrated phenotypic measures sex-specific responses including enrichment mTOR signaling females, while males displayed enhanced mitochondrial ribosome biogenesis oxidative metabolism. Overall, this study reinforces our understanding impacts biology provides rich resource interrogate