Investigating mitochondrial gene expression patterns inDrosophila melanogasterusing network analysis to understand aging mechanisms

• DOI: 10.1101/2023.05.16.540914
• Publication Date: 2023-05-18T17:40:11Z
• Authors: Manuel Mangoni; Francesco Petrizz...; Niccolò Liorni; Salvatore Daniele...; Tommaso Biagini; Alessandro Napoli; Marta Adinolfi; Pietro Hiram Guzzi; Antonio Novelli; Viviana Caputo; Tommaso Mazza
• Keywords: Drosophila melanogaster; network analysis; mitochondrial DNA; aging; Technology; QH301-705.5; T; Physics; QC1-999; aging; mitochondrial DNA; Engineering (General). Civil engineering (General); Chemistry; <i>Drosophila melanogaster</i>; network analysis; mitochondrial DNA; aging; TA1-2040; Biology (General); <i>Drosophila melanogaster</i>; network analysis; QD1-999

AbstractThe process of aging is a complex phenomenon that involves a progressive decline in physiological functions required for survival and fertility. To better understand the mechanisms underlying this process, the scientific community has utilized several tools. Among them, mitochondrial DNA has emerged as a crucial factor in biological aging, given that mitochondrial dysfunction is thought to significantly contribute to this phenomenon. Additionally,Drosophila melanogasterhas proven to be a valuable model organism for studying aging due to its low cost, capacity to generate large populations, and ease of genetic manipulation and tissue dissection. Moreover, graph theory has been employed to understand the dynamic changes in gene expression patterns associated with aging and to investigate the interactions between aging and aging-related diseases. In this study, we have integrated these approaches to examine the patterns of gene co-expression inDrosophila melanogasterat various stages of development. By applying graph-theory techniques, we have identified modules of co-expressing genes, highlighting those that contain a significantly high number of mitochondrial genes. We found important mitochondrial genes involved in aging and age-related diseases inDrosophila melanogaster, including UQCR-C1, ND-B17.2, ND-20, and Pdhb. Our findings shed light on the role of mitochondrial genes in the aging process and demonstrate the utility ofDrosophila melanogasteras a model organism and graph theory in aging research.