MiDAS 5: Global diversity of bacteria and archaea in anaerobic digesters

• DOI: 10.1101/2023.08.24.554448
• Publication Date: 2023-08-25T06:10:17Z
• Authors: Morten Kam Dahl D...; Kasper Skytte And...; Anne-Kirstine C. ...; Vibeke Rudkjøbing; Madalena Alves; Yadira Bajón-Fern...; Damien Batstone; Caitlyn Butler; Mercedes Cecilia ...; Åsa Davidsson; Leonardo Erijman; Christof Holliger; Konrad Koch; Norbert Kreuzinger; Changsoo Lee; Gerasimos Lyberatos; Srikanth Mutnuri; Vincent O’Flaherty; Piotr Oleskowicz-...; Dana Pokorna; Veronica Rajal; Michael Recktenwald; Jorge Rodríguez; Pascal E. Saikaly; Nick Tooker; Julia Vierheilig; Jo De Vrieze; Christian Wurzbacher; Per Halkjær Nielsen
• Keywords: Metagenomic Analysis; Wastewater; Gene; Microbial ecology; Bioreactors; Engineering; Environmental biotechnology; RNA, Ribosomal, 16S; Anaerobiosis; Phylogeny; Amplicon sequencing; Environmental microbiology; Ecology; SYNTROPHUS-ACIDITROPHICUS; MICROBIAL COMMUNITY; Microbiota; Q; Life Sciences; ASSOCIATION; Biodiversity; Diversity and Function of Gut Microbiome; ddc:; Polymerase chain reaction; GENOME; Ribosomal RNA; Physical Sciences; PROPIONATE OXIDATION; SP-NOV.; GEN. NOV.; FATTY-ACIDS; Anaerobic Digestion and Biogas Production; Methane; 16S ribosomal RNA; Microorganism; Science; SMITHELLA-PROPIONICA; Candidatus; Euryarchaeota; Article; Microbial Ecology; Biochemistry, Genetics and Molecular Biology; Anaerobic digestion; Genetics; Molecular Biology; Biology; Obesity-associated Microbiome; Bacteria; Marine Microbial Diversity and Biogeography; Anaerobic Digestion; Biology and Life Sciences; Sequence Analysis, DNA; Building and Construction; Amplicon; Archaea; Anaerobic bacteria; FOS: Biological sciences; Environmental Science; SP. NOV.; Biogas Production; Metagenomics; Microbiome

Abstract Anaerobic digestion represents a key biotechnology for the transformation of organic waste into renewable energy (biogas) and relies on complex microbial communities that work in concert to degrade substrates methane carbon dioxide. Here, we sequenced more than half million high-quality, full-length 16S rRNA gene sequences from 285 full-scale anaerobic digesters (ADs) across world expand our knowledge about diversity function bacteria archaea ADs. The were processed amplicon sequence variants (FL-ASVs), which added MiDAS 4 database wastewater treatment systems create 5. expansion significantly increased coverage ADs worldwide, leading an improved rate genus species-level classification. Using 5, carried out amplicon-based, global-scale community profiling sampled using three common sets primers targeting different regions and/or archaea. We revealed how environmental conditions biogeography shape AD microbiota. also identify core conditionally rare or abundant taxa, encompassing 692 genera 1013 species. These represent 84-99% 18-61% accumulated read abundance respectively, samples depending used. Finally, examined global functional groups with known importance process. Our online Field Guide presents data generated this study summarizes present all taxa.