The Pex6 N1 domain is required for Pex15 binding and proper assembly with Pex1

• DOI: 10.1101/2023.09.15.557798
• Publication Date: 2023-09-17T07:10:20Z
• Authors: Bashir A. Ali; Ryan M. Judy; Saikat Chowdhury; Nicole K. Jacobsen; Dominic T. Castanzo; Kaili L. Carr; Chris D. Richardson; Gabriel C. Lander; Andreas Martin; Brooke M. Gardner

The heterohexameric AAA-ATPase Pex1/Pex6 is essential for the formation and maintenance of peroxisomes. Pex1/Pex6, similar to other AAA-ATPases, uses energy from ATP hydrolysis mechanically thread substrate proteins through its central pore, thereby unfolding them. In related motors, substrates are recruited binding motor's N-terminal domains or N-terminally bound co-factors. Here we use structural biochemical techniques characterize function N1 domain in Pex6 budding yeast,