Bi-directional communication between monocytes and trophoblasts under hypoxia and hypoxia-reperfusion conditions

• DOI: 10.1101/2023.09.23.558721
• Publication Date: 2023-09-25T09:30:44Z
• Authors: Hannah Yankello; Yerim Lee; Christina Megli; Elizabeth Wayne
• Keywords: Monocyte; Trophoblast; Hypoxia; Decidua

Abstract Introduction Pregnancy-related disorders such as preeclampsia are associated with syncytiotrophoblast (STB) stress and monocyte dysregulation. It remains unclear whether this derives from prolonged placental hypoxia or a hypoxia-reperfusion-type injury. Thus, study investigated how these two models of STB impact trophoblast-monocyte interactions. Method Cobalt chloride chemically induced in BeWo b30 cells. A transwell coculture system was used to examine signaling. qPCR quantified gene expression changes following coculture. Monocyte phagocytosis E. Coli adhesion cells determined via flow cytometry. migration signals using cell counter. Results hypoxic state b30s. Reperfusion restored the indirect genes ER genes. Coculturing THP-1 monocytes normoxic, hypoxic, hypoxic-reperfused b30s promoted survival but not wound-healing capacity. Compared BeWos, increased inflammatory expression, decreased phagocytosis, did change migration. Finally, signaling early-onset PE chemotaxis, precondition more strongly influenced compared state. Discussion Overall, most effectively recapitulate functional behavior observed preeclampsia. Further research is needed understand spatial temporal monocyte-trophoblast interactions pregnancy outcomes. chemotaxis primary varied by gestational age, maternal diagnosis, condition, implying could be biomarkers predict their at maternal-fetal interface well onset disease. Figure