Formation of hemiclonal reproduction and hybridogenesis inPelophylax waterfrogs studied with species-specific cytogenomic probes

• DOI: 10.1101/2023.10.29.564577
• Publication Date: 2023-11-02T01:40:20Z
• Authors: Choleva Lukáš; Doležálková-Kaštá...; Labajová Veronika; Sember Alexandr; Altmanová Marie; Lukšíková Karolína; Chung Voleníková ...; Dalíková Martina; Nguyen Petr; Pustovalova Eleonora; Fedorova Anna; Dmitrij Dedukh
• Keywords: Sexual reproduction

ABSTRACT Meiosis is a conservative process in all sexual organisms which ensures fertility and central for producing genetic diversity by recombination random segregation of parental chromosomes. Yet unexplored mechanisms may disrupt it cause ‘loss sex’ followed the emergence clonal modes reproduction. Interspecific hybridization primary trigger this process, but mechanistic basis transition to asexuality remains still unknown most vertebrate animals. To study these processes water frogs, we performed reciprocal mating between two species, Pelophylax ridibundus P. lessonae , produced vital F1 progeny ( esculentus ). The RepeatExplorer2 analysis low-coverage genomic data species identified -specific minisatellite marker PlesSat01-48 (44 bp), hybridized (peri)centromeric regions chromosome pairs – acrocentric 8 10 (a carrier nucleolar organizer region; NOR). Chromosomal mapping combining novel probe with previously designed centromeric satellite DNA showed that do not reproduce sexually. Instead, generation instantly modified its gametogenesis established asexual reproduction via hybridogenesis. Gametogenic modifications included premeiotic elimination one genomes propagation remaining genome endoreplication standard meiotic division. origin hybridogenesis laboratory-produced hybrids supports hypothesis arises recurrently nature whenever come into reproductive contact. Based on observed pattern discuss evolution population systems frogs applicability newly chromosomal other taxa.