Streptococcus pneumoniaeaugments circadian clock gene expression in zebrafish cells

• DOI: 10.1101/2024.02.20.581283
• Publication Date: 2024-02-27T18:02:19Z
• Authors: Camila Morales Fé...; Raina E. Sacksteder; Jacqueline M. Kimmey
• Keywords: PER2; Danio

Abstract The circadian clock is a highly coordinated, cell-autonomous process that regulates the daily internal rhythms of biological organisms. Circadian clocks have been identified in various organisms, including mammals, invertebrates, cyanobacteria, and plants. zebrafish ( Danio rerio ) practical model for studying vertebrate due to its small size, ease manipulation, gene tractability, direct light responsiveness. Several studies revealed bacterial, viral, parasitic infections can impact expression genes mammals. While some evidence suggests this may also be case zebrafish, no investigated effects bacterial exposure on clock. Here, using Z3 cells, we show heat-killed Streptococcus pneumoniae (HK-Spn) augment core repressive factors, per1b, per2, per3, cry1a . Further investigation demonstrated HK-Spn induces production reactive oxygen species (ROS) cells addition NAC, ROS antioxidant, blocks Spn-mediated induction per2 , per3 Additionally, augmented tefa tefb, an effect NAC suppressed. These results suggest involvement ROS-dependent pathway augmentation cry1a, by HK-Spn. Moreover, activation tefb represents promising new targets further regulation these genes.