Unveiling Novel Biocontrol Strategies: Serratia marcescens chiA Gene Against Myzus persicae

• DOI: 10.1101/2024.06.29.601316
• Publication Date: 2024-06-30T06:30:12Z
• Authors: Ahmet Can; Ömür Baysal
• Keywords: Myzus persicae

Abstract Background Microorganisms produce a diverse array of enzymes with potential applications in biological control and pest management. Among these enzymes, chitinase stands out due to its safety for non-target organisms the environment. Chitinase play crucial role breaking down chitin, which serves as critical component insect exoskeletons fungal cell walls. As result, they emerge valuable tools managing both agricultural pests pathogens. Results The chiA gene region Serratia marcescens GBS19 was successfully amplified via PCR cloned into expression vectors. resulting protein expressed purified through His-tag affinity chromatography. enzyme exhibited optimal activity at 40 °C pH 5. insecticidal properties were tested against Myzus persicae , revealing an LD 50 value 15,804 ppm. Comparative analysis ref_seq demonstrated that our shows 98.93% similarity, indicating high degree conservation likely functional similarity. Bioinformatics modelling highlighted strong binding (-4.10 kcal/mol) between also confirmed modelled chitin layer interaction. Conclusion study underscores S. effective environmentally safe biocontrol agent. exhibits promising properties, specifically M. supports effectiveness. Given environment, holds significant promise tool integrated management, contributing sustainable practices using directed recombinant DNA technology.