Integrative multi-omics data elucidating the biosynthesis and regulatory mechanisms of furanocoumarins inAngelica dahurica

• DOI: 10.1101/2024.07.23.604792
• Publication Date: 2024-07-24T19:05:19Z
• Authors: Jiaojiao Ji; Xiaoxu Han; Lanlan Zang; Yushan Li; Liqun Lin; Donghua Hu; Shichao Sun; Yonglin Ren; Garth Maker; Zefu Lu; Li Wang

Abstract Furocoumarins (FCs) are crucial natural products playing a dual role as plant defense molecules and pharmacologically active substances. Angelica dahurica is renowned herb with diverse abundant FCs. However, the accumulation pattern over developmental stages, biosynthesis pathway regulatory mechanisms of FCs in A. remain elusive, hindering production via synthetic biology approaches. Here, we constructed chromosome-level reference genome for quantified content dynamics 17 coumarins across six stages its medicinal organ, root. It showed gradual decrease FC concentration root enlargement. The combined analyses transcriptomic metabolomic data, together vivo enzymatic assay, confirmed that CYP71AZ18 was involved bergaptol, whereas CYP71AZ19 CYP83F95 contributed to xanthotoxol. Notably, originated from proximal duplication event CYP71AZ18, specific , subsequently undergoing neofunctionalization. Accessible chromatin regions (ACRs), especially ACRs, correlated higher gene expression levels, including three validated genes biosynthesis, showing potential regulate metabolite biosynthesis. Our findings provide new insights into biosynthetic epigenetic regulation