Condensin-dependent chromatin condensation represses transcription globally during quiescence

• DOI: 10.1101/320895
• Publication Date: 2018-05-12T18:30:13Z
• Authors: Sarah G. Swygert; Seungsoo Kim; Xiaoying Wu; Tianhong Fu; Tsung-Han Hsieh; Oliver J. Rando; Robert N. Eisenman; Jay Shendure; Jeffrey N. McKnight; Toshio Tsukiyama
• Keywords: Condensin; ChIA-PET; Transcription

SUMMARY Quiescence is a stress-resistant state in which cells reversibly exit the mitotic cell cycle and suspend most cellular processes. essential for stem maintenance its misregulation implicated tumor formation. One of conserved hallmarks quiescent cells, from Saccharomyces cerevisiae to humans, highly condensed chromatin. Here, we use Micro-C XL map chromatin contacts at single-nucleosome resolution genome-wide elucidate mechanisms functions S. cells. We describe previously uncharacterized domains on order 10-60 kilobases that are formed by condensin-mediated loops. Conditional depletion condensin prevents condensation during quiescence entry leads widespread transcriptional de-repression. further demonstrate condensin-dependent compaction human fibroblasts. propose represses transcription throughout genome.