Reference genome for the WHO reference strain for Mycobacterium bovis BCG Danish, the present tuberculosis vaccine

• DOI: 10.1101/513408
• Publication Date: 2019-01-08T04:06:30Z
• Authors: Katlyn Borgers; Jheng-Yang Ou; Po-Xing Zheng; Petra Tiels; Annelies Van Hecke; Evelyn Plets; Gitte Michielsen; Nele Festjens; Nico Callewaert; Yao-Cheng Lin
• Keywords: 0301 basic medicine; 03 medical and health sciences; 3. Good health

ABSTRACTMycobacterium bovis bacillus Calmette-Guérin (M. bovis BCG) is the only vaccine available against tuberculosis (TB). This study reports on an integrated genome analysis workflow for BCG, resulting in the completely assembled genome sequence of BCG Danish 1331 (07/270), one of the WHO reference strains for BCG vaccines. We demonstrate how this analysis workflow enables the resolution of genome duplications and of the genome of engineered derivatives of this vaccine strain.