Chromosomal rearrangements at hypomethylated Satellite 2 sequences are associated with impaired replication efficiency and increased fork stalling

• DOI: 10.1101/554410
• Publication Date: 2019-02-19T17:35:21Z
• Authors: Yannick Delpu; Thomas McNamara; Patrick Griffin; Suhail Kaleem; Shubhada Narayan; Carl Schildkraut; Karen Miga; Mamta Tahiliani
• Keywords: 0303 health sciences; 03 medical and health sciences

AbstractCancer cells, aging cells, and cells from patients with the developmental disorder Immunodeficiency, Centromeric instability, and Facial anomalies (ICF) syndrome frequently display a striking loss of DNA methylation (hypomethylation) that is accompanied by increased DNA damage and chromosomal rearrangements. Despite the robust link, the mechanism by which hypomethylation leads to genomic instability is poorly understood. We report that the human pericentromeric repeat sequence Satellite 2 (SAT2) poses challenges to the DNA replication machinery when hypomethylated. Loss of methylation at SAT2 is associated with increased frequencies of chromosomal abnormalities and DNA damage. Hypomethylation of SAT2 is associated with elevated levels of replication stress signaling, and chromosomal abnormalities involving SAT2 are enhanced by low levels of aphidicolin-induced replication stress. To investigate the basis for these chromosomal abnormalities, we developed a single-molecule approach employing DNA combing to examine the progress of replication forks through SAT2 at the resolution of a single DNA molecule. Our analysis of replicating SAT2 molecules provides in vivo evidence that hypomethylation of SAT2 strongly decreases the efficiency of replicating these sequences suggesting that hypomethylation results in the formation of barriers to the replication machinery. Consistent with increased frequency of fork stalling at these sequences, we find increased levels of single-stranded DNA (ssDNA) binding protein RPA2 as well as asymmetric progression of sister replication forks within hypomethylated SAT2 sequences. Together these findings indicate that impaired replication triggers the formation of chromosomal aberrations observed at hypomethylated SAT2 sequences and also suggests a mechanistic basis for how the loss of DNA methylation may contribute to genomic instability in diverse pathological conditions.