The Gag Protein PEG10 Binds to RNA and Regulates Trophoblast Stem Cell Lineage Specification

• DOI: 10.1101/572016
• Publication Date: 2019-03-08T21:25:14Z
• Authors: Mona Abed; Erik Verschueren; Hanna Budayeva; Peter Liu; Donald S. Kirkpat...; Rohit Reja; Sarah K. Kummerfeld; Joshua D. Webster; Sarah Gierke; Mike Reichelt; Keith R. Anderson; Robert J Newman; Merone Roose-Girma; Zora Modrusan; Hazal Pektas; Emin Maltepe; Kim Newton; Vishva M. Dixit
• Keywords: Placentation; Trophoblast; Group-specific antigen; Genomic Imprinting; Retrotransposon

ABSTRACT Peg10 (paternally expressed gene 10) is an imprinted that essential for placental development. It thought to derive from a Ty3-gyspy LTR (long terminal repeat) retrotransposon and retains Gag Pol-like domains. Here we show the domain of PEG10 can promote vesicle budding similar HIV p24 protein. Expressed in subset mouse endocrine organs addition placenta, was identified as substrate deubiquitinating enzyme USP9X. Consistent with having critical role development, PEG10-deficient trophoblast stem cells (TSCs) exhibited impaired differentiation into lineages. wild-type, differentiating TSCs bound many cellular RNAs including Hbegf (Heparin-binding EGF-like growth factor), which known play important placentation. Expression reduced suggesting might bind stabilize are normal