Anti-microbial immunity is impaired in COPD patients with frequent exacerbations

• DOI: 10.1101/632372
• Publication Date: 2019-05-10T04:45:22Z
• Authors: Aran Singanayagam; Su-Ling Loo; Maria Calderazzo; Lydia J Finney; Maria-Belen Truji...; Eteri Bakhsoliani; Jason Girkin; Punnam Veerati; Prabuddha S Pathi...; Kristy S Nichol; Andrew Reid; Joseph Foottit; Sebastian L Johnston; Nathan W Bartlett; Patrick Mallia
• Keywords: Ex vivo

ABSTRACT Background Patients with frequent exacerbations represent a chronic obstructive pulmonary disease (COPD) sub-group requiring better treatment options. The aim of this study was to determine the innate immune mechanisms that underlie susceptibility in COPD. Methods We measured sputum expression mediators and bacterial loads samples from patients COPD at stable state during virus-associated exacerbations. Ex vivo responses rhinovirus infection differentiated bronchial epithelial cells (BECs) sampled were additionally evaluated. stratified as exacerbators (≥2 preceding year) or infrequent (<2 comparisons made between these groups. Results Frequent had reduced cell mRNA anti-viral type I III interferons interferon-stimulated gene (ISG) when clinically exacerbation. RV-induction interferon ISGs ex also impaired BECs exacerbators. levels anti-microbial peptide mannose-binding lectin (MBL)-2 an associated increase 2 weeks following exacerbation onset. MBL-2 correlated negatively Conclusion These data implicate deficient airway immunity increased propensity observed some Therapeutic approaches boost antimicrobial lung could be viable strategy for prevention/treatment