Genome improvement and genetic map construction for Aethionema arabicum, the first divergent branch in the Brassicaceae family

• DOI: 10.1101/662684
• Publication Date: 2019-06-07T04:25:12Z
• Authors: Thu-Phuong Nguyen; Cornelia Mühlich; Setareh Mohammadin; Erik van den Bergh; Adrian E. Platts; Fabian B. Haas; Stefan A. Rensing; M. Eric Schranz
• Keywords: Sequence assembly

Abstract Background The genus Aethionema is a sister-group to the core-group of Brassicaceae family that includes Arabidopsis thaliana and Brassica crops. Thus, phylogenetically well-placed for investigation understanding genome trait evolution across family. We aimed improve quality reference draft version annual species arabicum . Secondly, we constructed first Ae. genetic map. improved map enabled development each other. Results started with initially published (version 2.5). PacBio MinION sequencing together v2.5 were incorporated produce new v3.0. contains 203 MB sequence, approximately 94% assembly made up called bases, assembled into 2,883 scaffolds. N 50 (10.3 MB) represents an 80-fold over initial release. generated Recombinant Inbred Line (RIL) population was derived from two ecotypes: Cyprus Turkey (the genotype. Using Genotyping by Sequencing (GBS) approach, high-density 749 (v2.5) then 632 SNPs (v3.0) generated. integrated, thus greatly improving scaffolding 11 linkage groups. Conclusions show long-read data genetics are complementary, resulting in They will facilitate comparative mapping work also valuable resources investigate wide range life history traits