Insights into the bacterial profiles and resistome structures following severe 2018 flood in Kerala, South India

• DOI: 10.1101/693820
• Publication Date: 2019-07-06T02:05:15Z
• Authors: Soumya Jaya Divak...; Jamiema Sara Philip; Padma Chereddy; Sai Ravi Chandra ...; Akshay Jaya Ganesh; Jiffy John; Shijulal Nelson-S...
• Keywords: Resistome; Pathogenic bacteria; Enterococcus faecalis

Abstract Extreme flooding is one of the major risk factors for human health, and it can significantly influence microbial communities enhance mobility infectious disease agents within its affected areas. The flood crisis in 2018 was severe natural calamities recorded southern state India (Kerala) that economy ecological habitat. We utilized a combination shotgun metagenomics bioinformatics approaches understanding microbiome disruption dissemination pathogenic antibiotic-resistant bacteria on flooded sites. Here we report, altered bacterial profiles at sites having 77 different genera comparison with non-flooded mangrove settings. regions were heavily contaminated faecal contamination indicators such as Escherichia coli Enterococcus faecalis resistant strains Pseudomonas aeruginosa, Salmonella Typhi/Typhimurium , Klebsiella pneumoniae, Vibrio cholerae Staphylococcus aureus . resistome contains 103 genes, which 38% are encoded plasmids, where most them associated pathogens. presence 6 susceptibility to multiple antibiotics including ampicillin, chloramphenicol, kanamycin tetracycline hydrochloride confirmed post-flooded using traditional culture-based analysis followed by 16S rRNA sequencing. Our results reveal profile following devastating event elevated levels both bacteria. circulation raw sewage from waste treatment settings urban area might facilitate spreading genes.