Performance assessment of total RNA sequencing of human biofluids and extracellular vesicles

• DOI: 10.1101/701524
• Publication Date: 2019-07-14T22:34:02Z
• Authors: Celine Everaert; Hetty Helsmoortel; Anneleen Decock; Eva Hulstaert; Ruben Van Paemel; Kimberly Verniers; Justine Nuytens; Jasper Anckaert; Nele Nijs; Joeri Tulkens; Bert Dhondt; An Hendrix; Pieter Mestdagh; Jo Vandesompele
• Keywords: Extracellular Vesicles; Extracellular vesicles; Pseudogene

Abstract RNA profiling has emerged as a powerful tool to investigate the biomarker potential of human biofluids. However, despite enormous interest in extracellular nucleic acids, sequencing methods quantify total content outside cells are rare. Here, we evaluate performance SMARTer Stranded Total RNA-Seq method platelet-rich plasma, platelet-free urine, conditioned medium, and vesicles (EVs) from these We found be accurate, precise, compatible with low-input volumes able few thousand genes. picked up distinct classes molecules, including mRNA, lncRNA, circRNA, miscRNA pseudogenes. Notably, read distribution gene drastically differ among In conclusion, first show that can used for unbiased unraveling complete transcriptome wide range biofluids their vesicles.