Pyruvate Kinase M Links Glucose Availability to Protein Synthesis

• DOI: 10.1101/715086
• Publication Date: 2019-07-25T17:29:18Z
• Authors: Nevraj S. Kejiou; Lena Ilan; Stefan Aigner; Enching Luo; Ines Rabano; Nishani Rajakulen...; Hamed S. Najafabadi; Stephane Angers; Gene W. Yeo; Alexander F. Palazzo
• Keywords: PKM2

Abstract How human cells coordinate various metabolic processes, such as glycolysis and protein translation, remains unclear. One key insight is that enzymes have been found to associate with mRNAs, however whether these regulate mRNA biology in response changes cellular state unknown. Here we report the glycolytic enzyme, pyruvate kinase M (PKM), inhibits translation of 7% transcriptome elevated levels glucose pyruvate. Our data suggest presence pyruvate, PKM associates ribosomes are synthesizing stretches polyacidic nascent polypeptides stalls elongation step translation. PKM-regulated mRNAs encode proteins required for cell cycle may explain previous results linking regulation. study uncovers an unappreciated link between ribosome likely coordinates intake metabolites regulation synthesis cycle.