SIZ1 is a nuclear host target of the nematode effector GpRbp1 from Globodera pallida that acts as a negative regulator of basal plant defense to cyst nematodes

• DOI: 10.1101/725697
• Publication Date: 2019-08-07T20:49:07Z
• Authors: Amalia Diaz-Granados; Mark G. Sterken; Jarno Persoon; Hein Overmars; Somnath S. Pokhare; Magdalena J Mazur; Sergio Martin-Ram...; Martijn Holterman; Eliza C. Martin; Rikus Pomp; Anna Finkers-Tomczak; Jan Roosien; Abdenaser Elashry; Florian Grundler; Andrei J Petrescu; Geert Smant; Aska Goverse
• Keywords: Globodera pallida; Plant Immunity; Nematode infection

Abstract Soil-borne cyst nematodes are obligatory sedentary parasites that cause severe losses to cultivation of major crops such as potato and soybean. Cyst establish specialised permanent feeding sites within the roots their host by manipulating plant morphology physiology through secreted effectors. Here we identified targets effector GpRbp-1 studied roles in plant-nematode interactions. was found interact yeast planta with Arabidopsis homologues Siz/PIAS-type E3 SUMO ligase SIZ1. Our results show a pathogen master regulator SIZ1 cells, which has not been demonstrated earlier our knowledge. The interaction localizes nucleus, suggesting nuclear functions immunity may be modulated GpRbp-1. Furthermore, nematode infection assays transcriptomic profiling indicate is required for susceptibility nematodes. So, these data ligases play an important role Based on prediction acceptor GpRbp-1, model proposed recruit SUMOylated full functionality cells. Significance statement can target SIZ1, key protein involved regulating several aspects biology, most likely manipulate SUMOylation proteins successful plants.