A comparative study of the capacity of mesenchymal stromal cell lines to form spheroids

• DOI: 10.1101/834390
• Publication Date: 2019-11-08T16:47:43Z
• Authors: Margaux Deynoux; Nicola Sunter; Elfi Ducrocq; Hassan Dakik; Roseline Guibon; Julien Burlaud-Ga...; Lucie Brisson; Louis-Romée le Nail; Olivier Hérault; Jorge Domenech; Philippe Roingeard; Gaëlle Fromont; Frédéric Mazurier
• Keywords: Spheroid; Ex vivo

Abstract Mesenchymal stem cells (MSCs)-derived spheroid models favor maintenance of stemness, ex vivo expansion and transplantation efficacy. Spheroids may also be considered as useful surrogate the hematopoietic niche. However, accessibility to primary cells, from bone marrow (BM) or adipose tissues, limit their experimental use lack consistency in methods form spheroids affect data interpretation. In this study, we aimed create a simple model by examining ability cell lines, human (HS-27a HS-5) murine (MS-5) BM origins, spheroids, compared MSCs (hMSCs). Our protocol efficiently allowed formation all types within 24 hours. Whilst hMSCs-derived began shrink after twenty-four hours, size derived lines remained constant during three weeks. The difference was partially explained balance between proliferation death, which could triggered hypoxia induced oxidative stress. results demonstrate that, unlike hMSCs, MSC make reproductible that are easily handled. Thus, help understanding mechanisms involved functions provide study interactions