Rejuvenating senescent and centenarian human cells by reprogramming through the pluripotent state

• DOI: 10.1101/gad.173922.111
• Publication Date: 2011-11-04T19:09:31Z
• Authors: Laure Lapasset; Ollivier Milhavet; Alexandre Prieur; Emilie Besnard; Amelie Babled; Nafissa Aït-Hamou; Julia Leschik; Franck Pellestor; Jean-Marie Ramirez; John De Vos; Sylvain Lehmann; Jean-Marc Lemaitre
• Keywords: Reprogramming; Centenarian; Senescence; Regenerative Medicine

Direct reprogramming of somatic cells into induced pluripotent stem (iPSCs) provides a unique opportunity to derive patient-specific with potential applications in tissue replacement therapies and without the ethical concerns human embryonic (hESCs). However, cellular senescence, which contributes aging restricted longevity, has been described as barrier derivation iPSCs. Here we demonstrate, using an optimized protocol, that senescence is not limit age-related physiology reversible. Thus, show our iPSCs generated from senescent centenarian have reset telomere size, gene expression profiles, oxidative stress, mitochondrial metabolism, are indistinguishable hESCs. Finally, centenarian-derived able redifferentiate fully rejuvenated cells. These results provide new insights iPSC technology pave way for regenerative medicine aged patients.