Parallel pathways of gene regulation: homologous regulators SWI5 and ACE2 differentially control transcription of HO and chitinase.

• DOI: 10.1101/gad.6.1.93
• Publication Date: 2007-06-05T21:15:46Z
• Authors: P R Dohrmann; G Butler; K Tamai; S Dorland; J R Greene; D J Thiele; D J Stillman
• Keywords: Transcription

Two independent pathways of transcriptional regulation that show functional homology have been identified in yeast. It has demonstrated previously SWI5 encodes a zinc finger DNA-binding protein whose transcription and cellular localization both are cell cycle regulated. We ACE2, region is nearly identical to SWI5, shows patterns cycle-regulated nuclear similar those seen for SWI5. Despite their similarities, ACE2 function separate regulation. activator the HO endonuclease gene, whereas not. In contrast, CTS1 gene (which chitinase), An additional parallel between SWI5/HO pathway ACE2/CTS1 regulated same way, require SWI4 SWI6 activators. Overproduction either or permits activation target from other pathway, suggesting proteins capable binding vivo promoters they do not usually activate. Chimeric SWI5/ACE2 fusion experiments suggest promoter specificity resides domain distinct domain.